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Comet assay comparison of different Corbicula fluminea (Mollusca) tissues for the detection of genotoxicity Genet. Mol. Biol.
Rigonato,Janaina; Mantovani,Mário Sérgio; Jordão,Berenice Quinzani.
The comet assay was used to study the sensitivity of the widely distributed freshwater bivalve mollusk Corbicula fluminea to the DNA-damaging alkylating-agent methylmethane sulfonate (MMS). This study was undertaken to ascertain if C. fluminea is a good bioindicator of pollutants in aquatic environments and identify which C. fluminea tissue is most effective and practical for genotoxicity studies. The mollusks were exposed to 0.6, 1.2 or 2.4 X 10-4 M MMS for 40 min and their hemolymph, gill tissue and digestive gland tissue assessed for the level of DNA damage and the time needed for the tissues to recovery. Regression analysis showed a direct linear dose-response relationship between MMS concentration and the number of damaged cells for hemolymph and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corbicula fluminea; Hemolymph; Gills; Digestive gland; Comet assay; Biomonitoring.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300023
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Temporal assessment of microbial communities in soils of two contrasting mangroves BJM
Rigonato,Janaina; Kent,Angela D.; Gumiere,Thiago; Branco,Luiz Henrique Zanini; Andreote,Fernando Dini; Fiore,Marli Fátima.
ABSTRACT Variations in microbial communities promoted by alterations in environmental conditions are reflected in similarities/differences both at taxonomic and functional levels. Here we used a natural gradient within mangroves from seashore to upland, to contrast the natural variability in bacteria, cyanobacteria and diazotroph assemblages in a pristine area compared to an oil polluted area along a timespan of three years, based on ARISA (bacteria and cyanobacteria) and nifH T-RFLP (diazotrophs) fingerprinting. The data presented herein indicated that changes in all the communities evaluated were mainly driven by the temporal effect in the contaminated area, while local effects were dominant on the pristine mangrove. A positive correlation of community...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangrove; Cyanobacteria; Diazotrophs; Oil contamination; NifH T-RFLP; ARISA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100087
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